Effects of the Hexane Extract from Glycosmis parva on LPS-induced Macrophage Activation
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Abstract
The in vitro effect of the hexane extract from branches of Glycosmis prava on LPSinduced
macrophage activation was investigated. Murine macrophages, J774A.1cells, were
used in this study. The extract suppressed nitric oxide production from LPS-activated
J774A.1 cells in a concentration dependent manner with IC50 44.70 μg/ml. It decreased the
mRNA expression of the inducible nitric oxide synthase (iNOS) which is responsible for NO
production as well as the cyclo-oxygenase 2 (COX-2) which is responsible for prostaglandins
(PGs) synthesis in these activated macrophages. The extract also inhibited the mRNA
expression of pro-inflammatory cytokines, including TNF-α, IL-1β and IL-6. The results
from this study indicated that the hexane extract from G. parva may have anti-inflammatory
activities against activated macrophages which play a significant role during inflammatory
process.
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