Nitric Oxide Inhibitory Activity of Thai Medicinal Plants Called Kod-Tung-Ha

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Sunita Makchuchit
Arunporn Itharat

Abstract

Thai herbal medicine called Kod-Tung-Ha composes of five plants: Angelica
dahurica, Angelica sinensis, Artemisia annua, Atractylodes lancea and Ligusticum sinense.
These plants were examined for their inhibitory activities against lipopolysaccharide (LPS)
induced nitric oxide (NO) production in RAW 264.7 cell lines. These plant extracts were also
tested for the inhibitory effect on LPS-induced TNF-α release in RAW 264.7 cell lines. The
extraction methods imitated by Thai folk doctors using such as maceration in ethanol or
ethanolic extract and boiling by water or water extract. The results were found that the
ethanolic extract of Atractylodes lancea exhibited the most potent inhibitory activity nitric
oxide production (IC50 = 9.70 μg/ml) an also possessed potent activity against TNF-α release
with an IC50 value of 24.35 μg/ml., followed by an ethanolic extract of Angelica sinensis
(IC50 = 12.52 μg/ml). The water extract of all plants were apparently inactive. These results
can support the use of these plants in combination as Kod-Tung-Ha for treatment of
inflammatory-related diseases through the inhibition of NO and TNF-α release.

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Section
2010 Annual Meeting Abstracts/Lectures