Inhibitory Effect of Derris reticulata Ethanol Extract on LPS-induced Macrophage Activation
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Abstract
Derris reticulata Craib is a plant in Leguminosae family which has flavonoids as its
major active compounds similar to other plants in genus Derris. It has been used as
expectorant and thirst relief. In this study, we studied the effect of ethanol extract from stem
of D. reticulata on LPS-induced macrophage activation. Murine macrophage, J774A.1 cells,
were pretreated with 6.25-100 μg/ml the extract for 24 h and then stimulated with 100 ng/ml
lipopolysaccharide (LPS) for 24 h. The extract inhibited nitric oxide production in LPSactivated
J774A.1 cells in concentration-dependent manner with IC50 at 62.5 μg/ml. The
effect of this extract on phagocytosis activity of LPS-activated J774A.1 cells was also
investigated. The extract at the concentrations of 50 and 100 μg/ml significantly inhibited
zymosan phagocytosis of LPS-activated cells in a concentration dependent manner. These
results demonstrated that flavonoids from D. reticulata may have potential to be an
inflammatory agent.
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