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Fasciolosis caused by Fasciola gigantica is an important disease in cattle and
buffaloes in Thailand. At present, an efficient molecular diagnostic test for fasciolosis is not
available. This study aims to produce monoclonal antibodies specific to two important
parasite antigens, cathepsin L and type 1 cystatin (FgStefin-1) and to evaluate their efficacy
as diagnostic tools. Purified native cathepsin L and recombinant cystatin produced in E. coli
will be used for immunization of BALB/c mice for three times in 3-week intervals using
Freund’s adjuvant. The spleen cells of the mice will be collected and fused with myeloma
cells to obtain clones that produce antigen-specific monoclonal antibodies. These monoclonal
antibodies will be analyzed for their sensitivity and specificity.
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