Genotyping of Polymorphic Marker (MSP3α and MSP3β) Genes of Plasmodium vivax Field Isolates from Malaria Endemic of Thailand
Main Article Content
Abstract
Two polymorphic marker genes; merozoite surface protein 3α (MSP3α) and
merozoite surface protein 3β (MSP3β) of Plasmodium vivax 100 field isolates have been
investigated using PCR-RFLP. Genotyping of PvMSP3α and PvMSP3β exhibited high
polymorphism in length and sequence. Three major types of PvMSP3α (Type A, B, and C)
and two major types of PvMSP3β (Type A, and B) were detected based on length of PCR
products. Fourteen alleles of both genes were distinguished by restriction fragment length
polymorphism, with difference frequencies. These results strongly support that P. vivax
isolates are highly diverse. PvMSP3α and PvMSP3β are reliable polymorphic markers for
population genetic analysis of P. vivax and PCR-RFLP provide a powerful method for
genotyping and identification of mixed parasite infections without the need for sequencing
Article Details
Upon acceptance of an article, the Pharmacological and Therapeutic Society of Thailand will have exclusive right to publish and distribute the article in all forms and media and grant rights to others. Authors have rights to use and share their own published articles.