Pharmacological Characterization of the NMDA Receptor in the Human Platelet
Main Article Content
Abstract
N-methyl-D-aspartate (NMDA) roceptors have critical roles in excitatory synaptic
transmission, plasticity and excitotoxicity in the CNS. These receptors also have been
implicated in many physiological processes such as information processing; learning and
memory, and in pathological processes such as hypoxia, degenerative diseases and drug-addicted brain damage. More recently, NMDA receptors were found in non-neuronal tissues
such as bone, pancreas and skin. This study aimed to investigate NMDA receptor in human
platelet. By using platelet aggregation study, the MK801 which is a non-competitive
antagonist in the channel of NMDA receptor can inhibit platelet aggregation induced by ADP
around 40-50%. By using radioligand binding study with centrifugation technique, [3H]
MK801 can bind to platelet with high affinity (Kd 27.99+/-6.12 nM, Bmax 888.76 +/-67.95
fmol/mg protein). The displacement of 0.5 nM [3H] MK801 in platelet by channel blockers
was monophasic (rank order: MK801> memantine> ketamine). In this study, binding
properties of NMDA receptor of platelet were compared to rat brain (Kd 1.308+/-0.13 nM,
Bmax 3075.33+/-112.86 fmol/mg protein) and rank order of channel blockers displacement
were MK801> ketamine > memantine. This technique is applied to study the role of native
human NMDA receptor as a marker of brain damage in drug-addicted patient.
Article Details
Upon acceptance of an article, the Pharmacological and Therapeutic Society of Thailand will have exclusive right to publish and distribute the article in all forms and media and grant rights to others. Authors have rights to use and share their own published articles.