Effects of Chitosan Extracted fromChanneled Apple Snailon Enhancing Growth Rate of Green Banana (Cavendish) by Using Tissue Culture Technique

Abstract

There were 3 objectives of this research. Firstly, to study the optimum quantity of Chitosan extract to enhance growth of green banana (Cavendish) by using tissue culture technique, numbers and lengths of shoots and roots were compared. Secondly, to compare the efficiency of Chitosan extract and Benzylaminopurine (BA) in enhancing number and length of shoot.Thirdly, to compare the efficiency of Chitosan extract andNaphthaleneacetic acid (NAA) in enhancing number and length of root. The Murashige and Skoog (MS) was used as a tissue culture medium. Independent T-test was used to analyze to two groups of the experiment data. The results showed that 1 ml. of Chitosan extract was the best quantity to promote the growth of shoot, average 4.33 shoots were obtained, 2 ml. of Chitosan extract was the best quantity to stimulate the length of shoot, average 4.33 cm. of shoot length were obtained, and 5 ml of Chitosan Extract was the best quantity to increase the number of root, average 9.33 roots were obtained with the average root length of 3.46 cm. The number of shoot when using Chitosan Extract and BA 1 ml were 5.00 and 6.00, respectively, the two groups of experiment were not significant different (p>0.05). The number of root when using Chitosan Extract and NAA 5 ml. were 9.33 and 8.33, respectively, the two groups of experiment were not significant different (p>0.05). The experiment results showed that Chitosan extract can be applied instead of BA and NAA to culture green banana