Effects of Freezing Extender Osmolality on Post-Thaw Semen Quality and Fertility of Thai Red Junglefowl Semen
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Abstract
The efficiency of frozen semen storage in chicken depends on the osmolality of the extender. The objective of this research was to compare the osmolality of the Thai red junglefowl extenders: low (LTRJFE), normal (TRJFE), and high (HTRJFE) osmolality levels with the Schramm extenders for frozen Thai red junglefowl semen. The experimental design used was the completely randomized design with 4-treatments and 12 replications. Then the quality of extenders was tested in Thai red junglefowl semen of 25 one-year-oldmales, by measuring the sperm motility after thawing with a computer-assisted semen analysis. The fertility of Thai red junglefowl semen was tested for 4 treatments and 6 replications with 6-hens/each. Thetotal number of hen used in this experiment was 144-hens. The results showed that the osmolarities of Schramm, LTRJFE, TRJFE, and HTRJFE were 441, 365, 452, and 472 mOsmol kg–1, respectively. The total motility after thawing frozen semen of the TRJFE (61.08±0.48%) was the best, highly significant different (P≤0.01) from the Schramm (54.83±0.52%), HTRJFE (37.83±0.66%), and LTRJFE (31.67±0.58%). The sperm progressive motile after thawing frozen semen of the TRJFE (24.50±0.79%) was the best, significantly different (P≤0.05) from the Schramm (23.25±0.58%), HTRJFE (21.83±1.04%), and LTRJFE (21.00±0.83%). The fertility rates of the TRJFE (57.00±0.70%) and Schramm (54.22±0.68%) were the best, highly significant different from the HTRJFE (32.90±1.74%) and LTRJFE (31.02±2.17%) (P<0.01). Therefore, the TRJFE was the optimum extenderfor the cryopreservation of Thai red junglefowl semen.
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