The amount of Husk and Straw along with Rubber Tree Sawdust for Substrate to Culturing Bhutanese Oyster Mushroom (Pleurotus pulmonarius)

Main Article Content

Karun Phungbunhan
Nuttakorn Intaravicha

Abstract

This study investigated the use of different mixtures of Rice husk and rice straw, along with rubber tree sawdust, for the cultivation of Bhutanese oyster mushroom (Pleurotus pulmonarius) through a Completely Randomized Design (CRD) with 5 treatments and 3 replications. The treatments included: 1. rubber tree sawdust 100%(control) 2. rubber tree sawdust 50 : rice husk 50% 3. rubber tree sawdust 80 : rice husk 20% 4. rubber tree sawdust 80 : rice straw 20% 5. rubber tree sawdust 33.3 : rice husk 33.3 : rice straw 33.3%. The results showed significant differences in mycelial growth, number of harvests, fresh weight, and length of stalk among the treatments. Treatment 2 (rubber tree sawdust 50: rice husk50%) exhibited the fastest full colonization time at 19.86 days, while Treatment 5 (rubber tree sawdust 33.3: rice husk33.3: rice straw 33.3%) took the longest at 22.91 days. Treatment 3 (rubber tree sawdust 80: rice husk20%) yielded the highest number of harvests, while Treatment 5 (rubber tree sawdust 33.3: rice husk33.3: rice straw 33.3%) yielded the least. For fresh weight, Treatment 3 (rubber tree sawdust 80: rice husk20%) had the highest yield, followed by Treatments 1, 4, 2, and 5 in descending order. Regarding the length of the stalk, Treatment 3 (rubber tree sawdust 80: rice husk20%) had the longest stalks, followed by Treatments 1, 4, 5, and 2. In conclusion, the study suggests that Bhutanese oyster mushroom can be cultivated using a substrate mixture of 80% rubber tree sawdust and 20% Rice husk or 100% rubber tree sawdust without significant differences in yield compared to using 100% rubber tree sawdust alone.

Article Details

How to Cite
Phungbunhan, K. ., & Intaravicha, N. . (2024). The amount of Husk and Straw along with Rubber Tree Sawdust for Substrate to Culturing Bhutanese Oyster Mushroom (Pleurotus pulmonarius). Journal of Vocational Education in Agriculture, 8(2), 135–145. retrieved from https://li01.tci-thaijo.org/index.php/JVIA/article/view/262238
Section
Research Article

References

Thonghem, R. & Chaichuchote, S. (2015). Improvement of Pleurotus sp. Strains from Bhutan by Dikaryon-Monokaryon Mating. Available from https://www.doa.go.th. Accessed date: 27 January 2000. (in Thai)

Panjikkaran, S. T., & Mathew, D. (2013). An environmentally friendly and cost effective technique for the commercial cultivation of oyster mushroom (Pleurotus florida (Mont.) Singer). Journal of the Science of Food and Agriculture, 93(4), 973-976.

Kalac, P. (2009). Chemical composition and nutritional value of European species of wild growing mushrooms. Food Chemistry, 113(1), 9-16.

Urben, A. F. (2004). Mushroom production using modified Chinese technology. Brasília: Embrapa. (in Portuguese)

Patil, S. S., et al. (2010). The nutritional value of Pleurotus ostreatus (Jacq.: Fr.) Kumm cultivated on different lignocellulosic agrowastes. Innovative Romanian Food Biotechnology, 7, 66-76.

Oyetayo, V. O., & Ariyo, O. O. (2013). Micro and macronutrient properties of Pleurotus ostreatus (Jacq: Fries) cultivated on different wood substrates. Jordan Journal of Biological Sciences, 6(3), 223-226.

Nakeesin, W. (2013). Use of spent coffee grounds as sawdust replament in tee oyster mushroom (Pleurotus ostreatus (Jacq.ex.Fr) Kummer) cultivation (Master thesis, Silpakorn University). (in Thai)

Nukpook, K., et al. (2019). Development of Screw Press for Packing Corn Husk Substrate in Long Polytene Bag for Mushroom Cultivation. Thai Agricultural Research Journal, 37(1), 37-47. (in Thai)

Ahmed, M., et al. (2013). Yield and nutritional composition of oyster mushroom strains newly introduced in Bangladesh. Pesquisa Agropecuária Brasileira, 48(2), 197-202.

Hussain, et al. (2002). Effect of different sterilization methods on the production of oyster mushroom (Pleurotus ostreatus) on different substrates. In The 3rd National Conference of Plant Pathology, NARC (p. 159–160). 1–3 October, 2001, Islamabad, Pakistan.

Namee, J & Seak, P. (2023). Utilization of Local Agricultural Wastes for the Production of Oyster Mushrooms (Pleurotus osteatus). Maejo Journal of Agricultural Production, 5(1), 203-215. (in Thai)

Okigbo, R. N., et al. (2021). Effect of using rice husk for cultivation of Pleurotus tuberregium (Fr.) Singer. Journal of Basic and Applied Sciences International, 27(5), 1-10.

Office of Agricultural Economics. (2019). Area of agricultural use in 2019. Available from https://www.oae.go.th. Accessed date: 29 January 2024. (in Thai)

Salmones, D., et al. (1999). Cultivation of shiitake mushroom, Lentinula edodes, in several lignocellulosic materials originating from the subtropics. Agronomie, 19(1), 13-19.

Obodai, M., et al. (2003). Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by-products. Journal of Industrial Microbiology & Biotechnology, 30(3), 146-149.

Melanouri, E. M., et al. (2022). Cultivating Pleurotus ostreatus and Pleurotus eryngii mushroom strains on agro-industrial residues in solid-state fermentation. Part II: Effect on productivity and quality of carposomes. Carbon Resources Conversion, 5(1), 52-60.

Srijumpa, N. & Khayankan, S. (2011). Agricultural Wastes as Substrates for Mushroom Cultivation. Thai Agricultural Research Journal, 29(2), 108–118. (in Thai)

Rupitak, Q., et al. (2016). Study on Mycelial Growth and Yield of Bhutanese Oyster Mushroom (Pleurotus pulmonarius) by Using Bagasse and Ground Bagasse as Material. Songklanakarin Journal of Plant Science, 3(Suppl.3), M11/48-53. (in Thai)

Kongkaewand, T. & Wattanakosol, A. (2021). Profit Alternative Material: The Manila Grass Ratio of Oyster Mushroom (Pleurotus ostreatus) Cultivated Materials. Journal of Science and Technology, Ubon Ratchathani University. 23(2), 74-80. (in Thai)

Costa, A. F. P., et al. (2023). The use of rice husk in the substrate composition increases Pleurotus ostreatus mushroom production and quality. Scientia Horticulturae, 321, 112372.

Payapanon, A ., et al. (2007). Pimgarn Arampongphan Sompong Angkhoram. Suitable amount of substrate for producing Lentinus polychrous Lev. in plastic bag. In The 44th Kasetsart University Annual Conference (p.571-578). 30 January – 2 February, 2006, Bangkok, Thailand. (in Thai)

Royse, D. J. (2002). Influence of spawn rate and commercial delayed release nutrient levels on Pleurotus cornucopiae (oyster mushroom) yield, size, and time to production. Applied Microbiology and Biotechnology, 58(5), 527-531