Antibacterial Activity of Honey from Bee (Apis cerana) and Candy Product Development

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Patcharee Lungmann
Chuanpit Ruangcharus
Weena Jirattiwarutkul Chaisarn
Dorkrak Chaisarn
Miti Jearaphunt
Sakchai Kamarangul
Cheeranan Klomnara Kaewruksa

Abstract

This research aimed to study the antibacterial properties of honey and develop honey candy which study physical and chemical properties of honey bee (Apis cerana) from Bee keeping Group, Pang Wan subdistrict, Phato district, Chumphon province. Honey bee was quantitative analyzed following the standard method. The results indicated that the honeybee could inhibit Escherichia coli and Staphylococcus aureus with the highest inhibition zone at 27±0.40 and 23±0.45 mm., respectively but it could not inhibit Bacillus subtilis. Various components of honey bee: Fructose and sucrose content were 309.46±2.75 and 296.04±26.89 mg/g, respectively. Glucose and xylose were not found. The nitrogen content as 0.034±0.00 percent (wt.). Ash exhibited the lowest content as 0.04±0.00 percent (wt.). Physical properties in 13 recipes of honey candy consist of L*, a*, b* were 28.71±0.01 to 40.98±0.04, 3.45±0.02 to 15.19±0.01, 0.98±0.02 to14.00±0.05, respectively. Hardness was 43.50±0.00 to 83.20±0.60 (no unit). The honey candy recipe for the highest customer satisfaction was 60 percent isomalt, 30 percent honey, 15 percent lemon juice, 0.5 percent starch and 0.2 percent salt.


 


 

Article Details

How to Cite
Lungmann, P., Ruangcharus, C. ., Jirattiwarutkul Chaisarn, W. ., Chaisarn, D. ., Jearaphunt, M. ., Kamarangul, S. ., & Klomnara Kaewruksa, C. . (2025). Antibacterial Activity of Honey from Bee (Apis cerana) and Candy Product Development. Journal of Vocational Education in Agriculture, 9(1), 91–99. retrieved from https://li01.tci-thaijo.org/index.php/JVIA/article/view/263896
Section
Research Article

References

Tafere, D. A. (2021). Chemical Composition and Uses of Honey: A Review. Journal of Food Science and Nutrition Research, 4(3), 194-201.

Engel, M. S., et al. (2019). A Key to the Genera and Subgenera of Stingless Bees in Indonesia (Hymenoptera: Apidae). Treubia, 45, 65–84.

Hilário, S. D.,et al. (2001). Responses to Climatic Factors by Foragers of Plebeia pugnax Moure (in litt.) (Apidae, Meliponinae). Brazilian Journal of Biology, 61(2), 191–196.

Bonev, B., et al. (2008). Principles of Assessing Bacterial Susceptibility to Antibiotics Using the Agar Diffusion Method. Journal of Antimicrobial Chemotherapy, 61(6), 1295-1301.

Kumar, D., et al. (2024). Honey: An Important Nutrient and Adjuvant for Maintenance

of Health and Management of Diseases. Journal of Ethnic Foods, 11, 19(2024).

Guiné, R. P. F., et al. (2022). Honey Bee (Apis mellifera L.) Broods: Composition, Technology and Gastronomic Applicability. Foods, 11(18), 2750.

Czernicka, M. A., (2024). Antioxidant Capacity of Honey Enriched by Wildflowers. Applied Sciences, 14(5), 2018.

Almasaudi, S. (2021). The Antibacterial Activities of Honey. Saudi Journal of Biological

Sciences. 28, 2188–2196.

AOAC. (2000). Official Methods of Analysis of the Association of Official Analytical Chemists. 15th ed. Virginia: Association of Official Analytical Chemists, Inc.

Veeranna, H., et al. (2021). Functional Hard-Boiled Candy Formulation Employing Plackett Burman Design. Indian Journal of Traditional Knowledge, 20(1), 230-236.

Anthimidou, E. & Mossialos, D. (2013). Antibacterial Activity of Greek and Cypriot Honeys Against Staphylococcus aureus and Pseudomonas aeruginosa in Comparison to Manuka Honey. Journal of Medicinal Food, 16(1), 42-47.

Badawy, O.F., et al. (2004). Antibacterial Activity of Bee Honey and Its Therapeutic Usefulness Against Escherichia coli O157:H7 and Salmonella typhimurium Infection. Revue scientifique et technique, 23(3), 1011–1022.

Wilkinson, J. M. & Cavanagh, H. M. A. (2005). Antibacterial Activity of 13 Honeys Against Escherichia coli and Pseudomonas aeruginosa. Journal of Medicinal Food, 8(1), 100–103.

Boukraa, L. & Niar, A. (2007). Sahara Honey Shows Higher Potency Against Pseudomonas aeruginosa Compared to North Algerian Types of Honey, Journal of Medicinal Food. 10(4), 712–714.

Kassym, L., et al. (2024). Antimicrobial Effect of Honey Phenolic Compounds Against E. coli—An In Vitro Study. Pharmaceuticals, 17, 560.

Kwakman, P. H. S. & Zaat, S. A. J. (2012). Antibacterial Components of Honey. IUBMB Life, 64(1), 48-55.

Ball, D. W. (2007). The Chemical Composition of Honey. Journal of Chemical Education, 84(10), 1643-1646.

Tsuruda, J. M., et.al. (2021). Honey Bee Nutrition. Veterinary Clinics of North America:

Food Animal Practice, 37(3), 505-519.

Sharma, K., et al. (2024). A Review of Physico-Chemical and Biological Properties of Honey. Journal of Entomology and Zoology Studies, 12(2), 153-161.

Rahardjo, M., et al. (2020). Color Development and Antioxidant Activity in Honey Caramel. IOP Conference Series: Earth and Environmental Science, 443, 012041.

Paravisini, L., et al. (2012). Identification of Compounds Responsible for the Odorant Properties of Aromatic Caramel. Flavour and Fragrance Journal, 27(6), 424–432.