Isolation of Pigment Producing Actinomycetes from Termite Mound in Sisaket Rajabhat University
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Abstract
The objectives of this research were to isolate the actinomycetes from a termite mound, study pigment production and extraction, and study cotton fiber dyeing with the extracted pigment. The pigment–producing actinomycetes were isolated from termite mound soil in Sisaket Rajabhat University by cultivating actinomycetes on Starch M-Protein Agar. Three isolates of pigment-producing actinomycetes on the substrate were obtained. They were circular and convex colonies, and the pigments appeared red, yellow, and orange. The pigment-producing actinomycetes were then inoculated on broken milled rice in solid-state fermentation for 3-5 days to study dye production. The inoculated broken milled rice was dried and ground. Dye was extracted with 70% ethanol for cotton fiber dyeing. Pink-color extracted dye from one isolate was able to dye cotton fibers. However, the dye should be compared with color standard specifications, and fiber dyeing, color fastness testing, and species identification of actinomycetes should be further investigated for dye application.
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