In Vitro Binding Effect of Yeast Byproduct on Aflatoxin B1

Abstract

Aflatoxin B1 (AFB1) is a fungal toxin produced by Aspergillus spp. This type of toxin has been evidently proved to cause hepatocellular carcinoma in various species of animals or even to human. Since a reduction of AFB1 has been concerned and large numbers of approaches are available, natural-based methods including using of?? bacteria and yeasts are ones that were adopted. In this research, yeast byproduct, which was discarded after fermentation of brewing, was used to adsorb AFB1 at pH 2 by incubating yeast byproduct concurrently with AFB1 at 37 °C for 24 hours. Thereafter, an extraction was completed by solid phase extraction (SPE) and AFB1 concentration?? was analyzed using HPLC-fluorescence detector. The results revealed that yeast byproduct had adsorptive capacity to adsorb AFB1, while percent binding was depended on yeast byproduct concentration. In details, percent binding of yeast byproduct concentrations of 5, 10, 25 and 50 mg/mL were 18.77%, 25.28%, 35.54% and 54.55%, respectively, for fixed concentration of 500 ng/mL of AFB1. The results showed a constant percent binding at 39.36 + 1.27% of 30 mg/mL of yeast byproduct to different concentrations of AFB1 (50, 100, 200, 300 and 500 ng/ml). As a result, yeast byproduct represented a capability of AFB1 reduction, which could be further utilized as a technique for aflatoxin decontamination.