Diversity of durian (Durio zibethinus L.) from Nonthaburi, Thailand based on morpho-palatability characteristics and simple sequence repeat markers

  • Wisuwat Songnuan Department of Plant Science, Faculty of Science, Mahidol University, Ratchathewi, Bangkok 10400, Thailand
  • Aussanee Pichakum Department of Plant Science, Faculty of Science, Mahidol University, Ratchathewi, Bangkok 10400, Thailand
  • Paweena Traiperm Department of Plant Science, Faculty of Science, Mahidol University, Ratchathewi, Bangkok 10400, Thailand
  • Em-orn Rungjangsuwan Department of Plant Science, Faculty of Science, Mahidol University, Ratchathewi, Bangkok 10400, Thailand
  • Umaporn Siriwattanakul Department of Plant Science, Faculty of Science, Mahidol University, Ratchathewi, Bangkok 10400, Thailand
  • Namkhang Leeratsuwan Department of Biology, Faculty of Science, Mahidol University, Ratchathewi, Bangkok 10400, Thailand
  • Piyarat Parinyapong Chareonsap Plant Genetic Conservation Project, Chitralada Villa, Dusit, Bangkok 10303, Thailand
  • Kasem Kulpradit Faculty of Environment and Resource Studies, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand
  • Songpol Somsri Horticultural Research Institute, Department of Agriculture, Ministry of Agriculture and Cooperatives, Chatuchak, Bangkok 10900, Thailand
  • Sasivimon Chomchalow Swangpol Department of Plant Science, Faculty of Science, Mahidol University, Ratchathewi, Bangkok 10400, Thailand
Keywords: Intraspecific variation, Morphometrics, Microsatellite markers, Phenogram, Simple sequence repeats (SSRs)

Abstract

Durian is a high-value, tropical fruit well known for its unique, pungent smell and sweet and creamy flesh. Nonthaburi province in central Thailand has been known as a source of top-quality durian cultivars, especially Mon Thong. However, with the recent urbanization and frequent severe weather conditions, these valuable local cultivars are disappearing at an alarming rate. Therefore, there is an urgent need for morpho-palatability as well as molecular information in order to correctly identify and characterize each cultivar. This study was the first to report durian diversity in Thailand using both morpho-palatability characteristics and molecular marker analysis. Morpho-palatability diversity was assessed in 31 cultivars using a modified descriptor for durian that measured 22 qualitative traits and 33 quantitative traits from the leaves, flowers and fruits. In addition, 24 cultivars were used for genetic diversity analysis based on simple sequence repeat (SSR) markers. The diversity of durian cultivars could be seen from the morphometric analysis. As expected, several cultivars showed unique characters that could be used for future breeding purposes. It was also found that certain characters were more informative than others at characterizing durian cultivars, which could lead to an improvement of cultivar identification. SSR marker profiles were considerably informative in distinguishing cultivars. Molecular data analysis showed that most cultivars were clustered together, with only a few in small, separate clusters. The results from this study should help to improve durian cultivar conservation and breeding for better quality.

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Published
2019-06-30
Section
Research Article