Cytological observation of anther development of cytoplasmic male sterility and thermosensitive genic male sterility systems in rice

  • Punyavee Dechkrong Central Laboratory and Greenhouse Complex, Research and Academic Service Center, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
  • Tanee Sreewongchai Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
  • Yupadee Paopun Scientific Equipment and Research Division, Research and Development Institute, Kasetsart University, Bangkok 10900, Thailand
  • Prapa Sripichitt Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
  • Fisseha Woreded Amhara Regional Agricultural Research Institute, Sirinka Agricultural Research Center, Bahia Dar, Ethiopia
Keywords: Anther development, Dysfunctional pollen, Male sterility, Pollen formation, Rice

Abstract

Male sterility is an excellent tool for use in hybrid rice production as it involves naturally occurring emasculation of the male gamete, thus preventing self-pollination in plant species. There are two male sterility systems—cytoplasmic male sterility (CMS) and thermosensitive genic male sterility (TGMS) —being widely used in rice breeding programs. Although TGMS and CMS systems are valuable tools for rice breeding, and the genetic and molecular mechanisms in various plants have been extensively studied, knowledge of the critical stage of cellular change in the rice anther is still imprecise. To gain an understanding of the actual crucial developmental stage of the rice anther that affects pollen productivity, cytological events were observed throughout the anther developmental process in both TGMS (KUT1) and CMS (IR80151A) rice lines. The results revealed that male sterility in the KUT1 line was most pronounced in the late meiotic stage during the developmental process and ultimately resulted in empty locules that were without pollen grains. In the IR80151A line, male sterility was displayed at the late vacuolated stage and consequently caused pollen abortion. Therefore, it was concluded that male sterility of CMS and TGMS occurs post meiosis.

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Published
2019-04-30
Section
Research Article