Optimization of reducing sugars production from agro-residue coconut leaflets using autoclave-assisted HCl hydrolysis with response surface methodology

Authors

  • Vinayaka Babu Shet Department of Biotechnology Engineering, NMAM Institute of Technology (V.T.U.Belagavi), Nitte, Udupi District, Karkala, 574110, Karnataka, India
  • Chetan Varun Department of Biotechnology Engineering, NMAM Institute of Technology (V.T.U.Belagavi), Nitte, Udupi District, Karkala, 574110, Karnataka, India
  • Aishwarya Uday Department of Biotechnology Engineering, NMAM Institute of Technology (V.T.U.Belagavi), Nitte, Udupi District, Karkala, 574110, Karnataka, India
  • Anusha Madu Palan Department of Biotechnology Engineering, NMAM Institute of Technology (V.T.U.Belagavi), Nitte, Udupi District, Karkala, 574110, Karnataka, India
  • Shama Umesh Rao Department of Biotechnology Engineering, NMAM Institute of Technology (V.T.U.Belagavi), Nitte, Udupi District, Karkala, 574110, Karnataka, India
  • Louella Concepta Goveas Department of Biotechnology Engineering, NMAM Institute of Technology (V.T.U.Belagavi), Nitte, Udupi District, Karkala, 574110, Karnataka, India
  • Selvaraj Raja Department of Chemical Engineering, Manipal Institute of Technology, Manipal Academy of Higher Education (MAHE), Udupi District, Manipal 576104, Karnataka, India
  • Chandrayan Vaman Rao Department of Biotechnology Engineering, NMAM Institute of Technology (V.T.U.Belagavi), Nitte, Udupi District, Karkala, 574110, Karnataka, India
  • Ujwal Puttur Department of Biotechnology Engineering, NMAM Institute of Technology (V.T.U.Belagavi), Nitte, Udupi District, Karkala, 574110, Karnataka, India

Keywords:

Acid hydrolysis, Coconut leaflets, Optimization, Reducing sugar

Abstract

The utilization of coconut leaflets, an agro residue generated from coconut farming, was investigated to develop a process to release reducing sugars using hydrochloric acid (HCl)-assisted, autoclave-based hydrolysis. The variables affecting HCl hydrolysis (duration of autoclaving, concentration of HCl and weight of coconut leaflets) were screened using a one factor at-a-time approach. The duration of autoclaving and concentration of HCl both had significant effects on the hydrolysis process and the levels of these factors were further optimized using a central composite design with response surface methodology. The optimized conditions were 3.17% of HCl and 44.14 min of autoclaving that released the maximum reducing sugars of 29 g/L. A second order model was generated, which was a good fit with a coefficient of determination value of 0.83.

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Published

2018-06-30

Issue

Section

Research Article