Soil extract calcium phosphate media for screening of phosphate-solubilizing bacteria

Authors

  • Manoharan Melvin Joe S. Thangapazham Agricultural College, Vasudevanallur, Tirunelvelli, 627758, India
  • Shalini Deivaraj Department of Microbiology, School of Life Sciences, VELS University, Chennai, 600117, India
  • Abitha Benson School of Biotechnology, School of Life Sciences, VELS University, Chennai, 600117, India
  • Allen John Henry Department of Microbiology, School of Life Sciences, VELS University, Chennai, 600117, India
  • G. Narendrakumar Department of Biotechnology, Faculty of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology, 600119, India

Keywords:

Aluminum phosphate Bacillus spp., Calcium phosphate, Rock phosphate, Phosphate solubilization

Abstract

Media development for screening bacteria capable of solubilizing inorganic complex forms of phosphorous to a soluble form is a major concern in agricultural research. This study formulated an effective, alternative solid and liquid media to the currently available Pikovskaya (PVK) medium, which relies on the use of calcium phosphate and numerous inorganic components for the screening of phosphatesolubilizing bacteria. In this present study, soil extract calcium phosphate (SECP) broth was formulated with simple medium requirements. SECP broth performed better than PVK broth as evidenced by its ability to support a higher bacterial population, higher reduction in broth pH and higher organic acid production and ultimately a better phosphate solubilization, based on the zone of clearance using bromocresol green or congo red in addition to bromothymol blue. Apart from calcium phosphate, rock phosphate and aluminum phosphate were also used as phosphate sources for the screening of different phosphate-solubilizing strains. This solid SECP medium could be used effectively with other phosphorous sources such as rock phosphate and aluminum phosphate. A high correlation between solid-based assay and broth-based assay for screening of phosphate-solubilizing bacteria was observed in the study.

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Published

2018-06-30

Issue

Section

Short Communication