Protective effect of Canna indica on cerebral ischemia-reperfusion injury in rats

Authors

  • Mallikarjuna Rao Talluri Bio Analytical Department, MicGene Lab, Visakhapatnam, Andhra Pradesh, 530003, India
  • Kishore Naidu Killari Department of Pharmacology, Sri Vishnu College of Pharmacy, Bhimavaram, Andhra Pradesh, 534201, India
  • N.V.S. Viswanadha Murthy Manepalli Department of Pharmaceutical Chemistry, AU College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, 530003, India
  • Prasad Konduri Department of Pharmacology, Sri Vishnu College of Pharmacy, Bhimavaram, Andhra Pradesh, 534201, India
  • Kiran Kumar Bandaru Department of Pharmacology, Sri Vishnu College of Pharmacy, Bhimavaram, Andhra Pradesh, 534201, India

Keywords:

Canna indica, Cerebral ischemia, Free radicals, Rhizome, Reperfusion

Abstract

The antioxidant capacity and cerebroprotective effect of Canna indica roots were estimated. The methanolic extract of C. indica roots was studied for free radical scavenging activity on superoxide, hydroxyl and 1, 1-diphenyl-2-picrylhydrazylfree radicals and compared with standard drug ascorbic acid using a pre-treatment for 1 wk at a daily oral dose, of 400 mg/kg or 800 mg/kg and then cerebral ischemiareperfusion injury was induced by occluding bilateral common carotid arteries for 30 min, followed by 4 h reperfusion. Quercetin (20 mg/kg, by intraperitoneal injection) was used as the standard drug. At the end of the experiment, animals were sacrificed by decapitation, and the brain was removed for the estimation of various biochemical parameters-assessment of cerebral infarct size and examination of oxidative stress enzymes such as superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and myeloperoxidase (MPO). Pre-treatment with methanolic extract of C. indica significantly reversed the levels of biochemical parameters and significantly reduced theedema and cerebral infarct size compared to the ischemic control group. The results indicated that C. indica ameliorates the cerebral ischemia/reperfusion injury and enhances the antioxidant defense. Further studies should involve the complete isolation of pure, biologically active compounds from the different extracts from C. indica like methanol in the present research.

Downloads

Download data is not yet available.

Downloads

Published

2017-12-31

Issue

Section

Research Article