Effects of Culture Media and Growing Media on Kluai Bep

Abstract

In vitro, culture of Kluai Bep was conducted in MS media supplemented with BA at 4 levels of concentration of 1,3,5 and 7 ppm. Subcultures were prepared every month. At high concentration of BA, more proliferation and shoot were obtained. Average proliferation time of BA cultured, on media of 1,3,5 and 7 ppm, were found to be 45, 41, 36 and 35 days respectively. It was also noticed that the numbers of shoot, increasing at the seventh month in 7 and 5 ppm BA, were 5.71 and 5.57, while those of 3 and 1 ppm BA 4.29 and 3.86 shoots/plant respectively. Shoots of 5 and 7 ppm BA were not significantly different from those of 1 and 3 ppm BA. All the plantlets were transferred to ¹/₂ MS without hormone for rooting. The survival rate of K. Bep after transplanting was 81.71 per cent. The horticultural characteristics such as height, size of plant, size of pseudostem and leaf were recorded after growing in 4 and 6 inches pots with different growing media. K. Bep growing in 4 inches pots with 2 parts soil mix : 2 parts of sand and 1 part of coir dust showed the best development on the ninth month according to the above character . Morphology of the 7^th generation of mature K. Bep were solid mutant of K.Khai.