Nucleotide sequencing of specific genes in nuclear and plastid genomes of good performance hybrids derived from interspecific hybridization in the genus Jatropha

Abstract

Biodiesel, produced from plant oil, has emerged as an attractive renewable energy source. Physic nut (Jatropha curcas L.) has gained widespread attention from both biodiesel producers and the research community due to its advantages of high biodiesel production, rapid growth, tolerance and convenient propagation. The current study used interspecific hybridization between J. curcas and Jatropha species and good performance hybrids DNA to study the relationship. Standardized DNA in the organism was used for DNA sequencing of the good performance hybrids as well as other Jatropha species. Sequencing was performed at four gene regions of the rbcL and trnH–psbA intergenic spacers in the plastid genome and the internal transcribed spacer of rDNA and stearoyl-acyl carrier protein desaturase (SAD) in the nuclear genome. Alignment of the SAD gene sequence showed high polymorphism among the five Jatropha species and hybrids. Phylogenetic trees based on the nucleotide sequences of regions could show that J. integerrima was closely related to J. curcas better than either gene.