Isolation and Characterization of Extracellular Halophilic Ribonuclease from Halotolerant Pseudomonas species

Authors

  • Werasit Kanlayakrit Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand
  • Takahiro Ikeda Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand
  • Somporn Tojai Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand
  • Mangkorn Rodprapakorn Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand
  • Sarote Sirisansaneeyakul Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand

Keywords:

halophilic, nuclease, ribonuclease, halotolerant, Pseudomonas sp.

Abstract

A halotolerant bacterium isolated from Thai fish sauce, obtained in Surathani province, was identified as a Pseudomonas sp. No. 3241. This strain showed halophilic ribonuclease activity. When casamino acids (CA) and yeast extract (YE) were used as the nitrogen source in a mini jar fermenter, a ratio concentration of CA to YE of 15:20 g/l in Sehgal and Gibbons Complex (SGC) medium, without NaCl, gave maximum growth and ribonuclease activity (18.18 U/ml). The ribonuclease enzyme was optimal at pH 10.0 and at the temperature of 50°C. It had marked halophilic enzyme properties that required an optimal NaCl concentration of 3 M. The ribonuclease was stable between pH 6.0 and 9.0 and at temperatures between 30 and 40°C.

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Published

2001-06-30

How to Cite

Kanlayakrit, Werasit, Takahiro Ikeda, Somporn Tojai, Mangkorn Rodprapakorn, and Sarote Sirisansaneeyakul. 2001. “Isolation and Characterization of Extracellular Halophilic Ribonuclease from Halotolerant Pseudomonas Species”. Agriculture and Natural Resources 35 (2). Bangkok, Thailand:179-87. https://li01.tci-thaijo.org/index.php/anres/article/view/240303.

Issue

Vol. 35 No. 2 (2001): April-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.