Screening and Identification of Effective Thermotolerant Lactic Acid Bacteria Producing Antimicrobial Activity Against Escherichia coli and Salmonella sp. Resistant to Antibiotics

Authors

  • Sunee Nitisinprasert Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand
  • Veeraphol Nilphai Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand
  • Phunjai Bunyun Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand
  • Prakit Sukyai Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand
  • Katsumi Doi Microbial Genetics Division, Institute of Genetic Resources, Kyushu University, 6-10-1 Hokozaki, Higashi-ku, Fukuoka, 812- 8581, Japan
  • Kenji Sonomoto Laboratory of Microbial Technology, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812, Japan

Keywords:

lactic acid bacteria, bacteriocin–like activity, antimicrobial activity, Lactobacillus reuteri

Abstract

Two hundred fifty six isolates of lactic acid bacteria (LAB) displaying antimicrobial activity were isolated from chicken intestine. Twenty effective isolates of LAB show bacteriocin – like activity (BLA) against the target strains resistant to antibiotics (Escherichia coli and Salmonella sp.) to varying degrees. Broad spectrum inhibition specific (SIS) were found from 6 h cultivation while the longer cultivation time of 12 and 24 h appeared either the loss of BLA or the inhibition activity against the different target strain. Only 3 effective thermotolerant isolates, i.e., KUB-AC5, KUB-AC16 and KUB-AC20, exerted broad SIS against all target strains except Salmonella sp. S001. However, BLA belonging to KUB-AC5 could not inhibit growth of E. coli E006 and E010 whereas KUB-AC20 did not inhibit only E. coli E006. Based on biochemical, physiological and molecular systematic method, these isolates were classified as Lactobacillus reuteri.

Downloads

Published

2000-09-30

How to Cite

Nitisinprasert, Sunee, Veeraphol Nilphai, Phunjai Bunyun, Prakit Sukyai, Katsumi Doi, and Kenji Sonomoto. 2000. “Screening and Identification of Effective Thermotolerant Lactic Acid Bacteria Producing Antimicrobial Activity Against Escherichia Coli and Salmonella Sp. Resistant to Antibiotics”. Agriculture and Natural Resources 34 (3). Bangkok, Thailand:387-400. https://li01.tci-thaijo.org/index.php/anres/article/view/240337.

Issue

Vol. 34 No. 3 (2000): July-September

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.