In Vitro Culture of Indica Rice ( Oryza sativa L. cv. Nang Mol S 4 )

Authors

  • Suriyun Cha-um Plant Tissue Culture Laboratory, National Center for Genetic Engineering and Biotechnology, Bangkok, 10400, Thailand
  • Srisom Surawattananon Department of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
  • Kramolpun Namwongprom Department of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
  • Chalermchai Wongwattana Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10100, Thailand

Keywords:

rice, Oryza sativa L., 2,4-dichlorophenoxyacetic acid, kinetin, indolebutyric acid

Abstract

Calli were induced from rice embryo ( Oryza sativa L. cv. Nang Mol S 4 ) on Murashige and Skoog ( MS ) supplemented with 2.0 mg/l 2,4-dichlorophenoxyacetic acid ( 2,4-D) in the dark condition. Proliferated calli were obtained on the MS supplemented with 2.0 mg/l 2,4-D in the same condition, Calli were regenerated on MS supplemented with 1.0 mg/l kinetin and 1.0 g/l casein hydrolysate under light condition. Roots were induced from shoots on the MS-free hormone or supplemented with indolebutyric acid ( IBA ) 0, 0.5, 1.0 or 2.0 mg/l). Servival of rice plants were 100 percentage after transplants under natural condition.

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Published

1997-06-30

How to Cite

Cha-um, Suriyun, Srisom Surawattananon, Kramolpun Namwongprom, and Chalermchai Wongwattana. 1997. “In Vitro Culture of Indica Rice ( Oryza Sativa L. Cv. Nang Mol S 4 )”. Agriculture and Natural Resources 31 (2). Bangkok, Thailand:166-74. https://li01.tci-thaijo.org/index.php/anres/article/view/240808.

Issue

Vol. 31 No. 2 (1997): April-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.