Growth and root formation of potato stem-bud segments cultured in vitro as influnced by 6-benzylamino purine and 6-furfurylamino purine.

Abstract

Stem-bud segments of potato (Solanum tuberosum L) ‘Apunta’ were cultured on Modified Murashige and Skoog (MS) (7) ager medium. The medium which consisted of MS macro and micro-elements, organic addenda and 0.05 ppm naphthaleneacetic acid (NAA) was supplemented with 6-benzylamino purine (BA) at 0.1, 0.2, 0.3, 0.4, 0.5 ppm (Experiment 1) or 6-furfurylamino purine (kinetin) 0.5, 1.0, 1.5, 2.0, or 2.5 ppm (Experiment 2). BA 0.1 ppm (Experiment 1) promoted the longest shoot and root and the highest number of leaves and roots, but tended to reduce number of branch. BA 0.5 ppm caused lesser number of branch and rroot to form than BA 0.1 ppm, while BA 0.4 ppm caused the least root formation. BA 0.3 ppm however promoted the most number of shoot branching. Kinetin 0.5 ppm (Experiment 2) promoted the longest shoot and the highest number of leaves and survival explants, but caused the least branching. Meanwhile, kinetin 2 ppm promoted the most number of branching.