Anther Culture of Rice (Oryza sativa L.)

Abstract

Anther of rice Oryza sativa L. spp. Indica cultivars RD1, RD3, RD5, RD7, RD 9, and RD11 at mid uninucleate microspore stage were cultured in vitro. The results are as follows : 1. Callus formation. Callus was formed best in N6 medium + 2 mg/1 2, 4-D + 3% sucrose. Increasing sucrose up to 6% decreased percentage of callus formation. Addition of 15% coconut water could not increasing percentage of callus formation. It was also found that genotype of plant has a great effect on callus formation. 2. Plantlet formation from callus. Green and albino plantlets were formed from callus in MS medium + 1 mg/1 NAA + 4 mg/1 kinetin + 3% sucrose. Using medium with 6% sucrose, the callus produced more green plantlets but less albino than those with 1.5% sucrose.