Sugarcane Tissue Culture : I. Influences of Growth regulators on Tissue Growth and Development

Abstract

Shoot tips, lateral buds and young which were cultured on media containing. Murashige and Skoog (MS) and macro and microelements, 1 or 3 ppm 2,4-Dichlorophenoxyacetic acid (2, 4-D) and 10% (v/v) young coconut water (CW) produced calli and shoots. Medium containing 3 ppm 2, 4-D, while medium containing no 2, 4-D did not or slightly promote callus formation. A6-furfurylamino purine (kinetin) at 0.1 ppm supplemented in the medium, instead of CW 10% (V/V), promoted callus and shoot formation but at a lesser extent than CW. Similarly, the effect of 3-Indoleacetic acid at 1 ppm could not replace the effect of 2, 4-D at 1 ppm whether CW or Kinetin was included or not. Meanwhile, sucrose at 30 gm per liter in the medium promoted better root formation and growth than sugar at 20 gm per liter.