Propagation of Amaryllis through Tissue Culture

Abstract

Bulb-scale sections of amaryllis (Hippestrum hybridum), 8 hybrids and one native variety, were cultured on modified Murashige and Skoog basal medium supplemented with 2,4 dichlorophenoxyacetic acid (2, 4-D) at 0.3 or 3 ppm combined with 6-furfurylamino purine (kinetin) at 0 or 0.3 ppm. Five varieties, Caroussel, Excelsior, Scarlet Leader, Rembrandt and Rilona, were able to regenerate bulblets. Excelsior tissues formed callus 90% and regenerate bulblet 50%. Both Rilona and Caroussel tissues formed callus 70% and bulblet 30%. Regeneration of bulblet in four varieties took 60-70 days but Caroussel variety took 32 days.