Callus induction and Growth of Zea mays. L var. saccharata (Super sweet corn) through Tissue Culture

Abstract

Callus induction of Zea mays var. saccharata (super sweet) on Murashige and Skoog (1962) medium containing 1 or 3 mg/1 2, 4-D was found. Tissue explants from root tip and mesocotyl were suitable for callus formation. The induction and growth of callus was better in medium containing 1 mg/1 2, 4-D and 10% coconut milk than medium containing 3 or mg/1 2, 4-D and coconut milk. Callus from root tip which was cultured in medium containing 1 or mg/1 2, 4-D grew at a greater extent under condition of light than in the dark. In contrast, callus from mesocotyl which was cultured in medium containing 1 mg/1 2, 4-D grew better under condition of dark than in the light. Nevertheless, callus grew better in the dark in medium which was supplemented with %coconut milk. The increasing percentage of callus growth was greater in medium containing 3 mg/1 2,4-D and 10% coconut milk or in coconut milk free-medium. Root formation occurred in callus which was cultured in medium containing 1 or 3 mg/1 2,4-D than in medium containing 1 mg/1 2,4-D than in medium containing 3 mg/1 2,4-D.