A Short Period Preservation of Sperm of Tawes, Puntius gonionotus (Bleeker)

Abstract

An experiment on a short period preservation of sperm of tawes (Puntius gonionotus) was conducted at Department of Aquaculture, Kasetsart University to find out the most suitable diluent using for sperm preservation. Tawes dry milt was devided into 3 parts, the first part was kept as dry milt; second was diluted with rinsing solution, and the rest was diluted with Ringer's solution, in a ratio of 5 parts of dry milt to 3 parts of diluent. All solutions plus dry milt were kept separately in small glass bottles with caps, placed in refrigerator, 4°C. After 24 and 72 hours each of sperm solution was mixed with freshly stripped eggs and fertility was estimated. A number of stripped eggs was also mixed with fresh milt stripped from alive male fish and used as control treatment. Fertilizing rates were 80.64% and 38.68% for milt diluted in Ringer's solution ; 60.17% and 3.71% for milt diluted in rinsing solution; 73.14% and 18.25% for undiluted dry milt; and 89.93% and 81.45% for fresh milt respectively. From statistical analysis, it appeared that tawes's milt can be preserved within 24 hours in Ringer's solution by keeping in refrigerator, 4°C. But for longer period the better diluent must be looked for.