Electron Microscopic Structure of White Blood Cells of Swamp Buffaloes (Bubalus Bubalis) I. Lymphocytes and Monocytes

Abstract

Lymphocytes and monocytes from the buffy coat of 10 adult swamp buffaloes (Bubalus bubalis) were studied. General feature of the lymphocytes was round or oval and the cell outline was slightly irregular. The nuclei were round or oval and had indentations. The cytoplasm contained abundant ribosomes. Two to ten round, oval or elongated mitochondria were seen. The small Golgi complex was situated near the nuclear indentation and was closely associated with the centrioles. There was a small quantity of endoplasmic reticulum while the number of azurophilic granules was quite variable. The monocytes were round or oval and were the largest among the cell types appearing on the electron microscope. The nuclei were lobule, round, oval or irregular round, in which consisted of fine chromatin granules, distributed compactly or loosely. A few to several dense round, oval or rod-shape granules were observed in the cytoplasm. Several round, oval or elongated mitochondria with clear cristae were seen. The centrioles and ill or well-developed Golgi complex were present near the nuclear indentation. A great number of smooth-surfaced endoplasmic reticulum were distributed on over the cytoplasm while a few rough-surfaced endoplasmic reticulum were also seen.