Anatomical Study of Corrosion Cast Kidney in the Swamp Buffalo

Abstract

Ten kidneys with renal arteries and ureters of swamp buffaloes (Bubalus bubalis) were collected from slaughter house. Arteries and ureters were injected with Technovit 7001 resin containing red pigment and yellow pigment, respectively. Place the specimens in cold water in a natural shape, and then corroded with 50% potassium hydroxide on next day. After one week, renal tissues were taken out in running water until thoroughly clean. Right and left renal arteries bifurcated into two main branches, cranial and caudal branches. The caudal branch gave off 1-2 middle branches. The two main branches and middle branch, then gave off interlobar branches to supply renal tissue. The cranial branch supplied the medial and lateral part of cranial pole, while caudal branch, the largest and longest, curved around lateral side of secondary branch ureter, then pass downward laterally to caudal pole and supplied both medial and lateral part of caudal pole. Middle branch supplied middle part of kidney and lateral border of cranial pole. Middle branch of left kidney was small due to the pointed cranial end. Ureter divided into two primary branches of ureter directed toward the poles of kidney and then subdivided to form the secondary branches of ureter which carrying calices, the funnel shaped structures. Number of renal calices were 27-53 and 23-49 in right kidney and left kidney, respectively. Small size calices were found in kidneys with high number calices. Serveral straight thread-like structures were found on the surface of some calices. These structures were no branches in SEM study, and represented collecting ducts. The pattern of primary, secondary branches of ureter and calices cast were correlated with the shape of kidney. This study is a basic background for understanding the pattern and distribution of renal arteries and renal calices of swamp buffaloes