Identification of Lime Cultivars and Hybrid by Isozyme patterns

Authors

  • Ariya Satrabhandhu Chitralada School, Bangkok 10303, Thailand.
  • Oradee Sahavacharin Department of Horticulture, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • Vichit Vangnai Department of Horticulture, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • Ravie Sethpakdee Department of Horticulture, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • Pradit Pongtongkam Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

isozymes, electrophoresis, limes, Citrus aurantifolia, interspecific hybrid

Abstract

Electrophoretic analysis of leaf extracts was used to identify lime cultivars and the interspecific hybrid of lime and leech lime. Both cold distilled water and phosphate buffer could be used as extraction buffer. In addition, the same isozyme patterns were obtained from the leaf extracts of three-month old cultured seedling and one-year-old potted plants. There was no difference in the peroxidase isozyme profiles in all cultivars of lime and leech lime tested. The esterase isozymes gave better discrimination in cultivars and inter specific hybrid characteristic. The observation of leaf morphology combined with isozyme analyses could differentiate between zygotic and nucellar seedlings after three-month growth in vitro.

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Published

1996-06-30

How to Cite

Ariya Satrabhandhu, Oradee Sahavacharin, Vichit Vangnai, Ravie Sethpakdee, and Pradit Pongtongkam. 1996. “Identification of Lime Cultivars and Hybrid by Isozyme Patterns”. Agriculture and Natural Resources 30 (2). Bangkok, Thailand:249-53. https://li01.tci-thaijo.org/index.php/anres/article/view/241154.

Issue

Vol. 30 No. 2 (1996): April-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.