Gene Transformation of Mungbean (Vigna radiata)

Authors

  • Surin Peyachoknagul Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Chareerat Phonjun Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Pradit Pongtongkam Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Paderm Ratisoontorn Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Saowanee Suputtitada Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Lertlak Ngernsiri Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

mungbean transformation, Vigna radiata, Agrobacterium mediated gene transfer

Abstract

Mungbean plants were subjected to Agrobacterium mediated transformation. Mungbean seedlings cultured on the modified MS medium for two days were wounded by cutting off one cotyledon. The recombinant plasmid containing a promoter (about 1 kb) of the WCI-2 gene fused with a coding region of a β-glucuronidase (GUS) gene was constructed. The pWCI-2 GUS fusion plasmid was then transferred to wounded mungbean seedlings by using Agrobacterium tumefaciens, The pBI121, a plasmid containing a CaMV 35S promoter fused with a GUS gene, was also used as a positive control. The putative transformed plants selected from kanamycin containing medium were tested for GUS activity and confirmed for the presence of transgene by PCR and Southern blot hybridization. The results showed that this method of gene transfer was possible for those plants with no regeneration system available.

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Published

1996-09-30

How to Cite

Surin Peyachoknagul, Chareerat Phonjun, Pradit Pongtongkam, Paderm Ratisoontorn, Saowanee Suputtitada, and Lertlak Ngernsiri. 1996. “Gene Transformation of Mungbean (Vigna Radiata)”. Agriculture and Natural Resources 30 (3). Bangkok, Thailand:303-11. https://li01.tci-thaijo.org/index.php/anres/article/view/241165.

Issue

Vol. 30 No. 3 (1996): July-September

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.