Effects of Prehydration, Sucrose Concentration and Developmental Stage of Anther on Pollen Germination of Chili cv. Kheenuu (Capsicum frutescens L. cv. Kheenuu)

Authors

  • Niran Juntawong Department of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Kanita Deeprala Department of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

pollen culture, Capsicum frutescens, prehydration, callose, osmoticum

Abstract

Pollen germination of Chili cv. Khwwnuu (Capsicum frutescens L. cv. Kheenuu) cultured in liquid medium comprising 100 ppm H3BO3, 300 ppm Ca(NO3)2, 200 ppm MgSO4.7H2O and 100 ppm KNO3 was studied. The result revealed that prehydration of pollen in a humid chamber for 30 min. significantly increased the germination percentage from 7.6 to 48.0. The medium with 15% sucrose (w/v) added had the best result for pollen germination. Moreover, pollen harvested from indehiscent anther germinated better than those from dehiscent anther. Fluorescene micrograph of pollen stained with 0.2% aniline blue fluorochrome showed the callosic layer of pollen tube.

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Published

1996-09-30

How to Cite

Niran Juntawong, and Kanita Deeprala. 1996. “Effects of Prehydration, Sucrose Concentration and Developmental Stage of Anther on Pollen Germination of Chili Cv. Kheenuu (Capsicum Frutescens L. Cv. Kheenuu)”. Agriculture and Natural Resources 30 (3). Bangkok, Thailand:373-77. https://li01.tci-thaijo.org/index.php/anres/article/view/241173.

Issue

Vol. 30 No. 3 (1996): July-September

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.