Malignant Hyperthermia in Swine I. Detection Comparing Between PCR and Halothane Technique

Authors

  • Sinchai Paraksa Department of Animal Science, Faculty of Agriculture, Kasetsart University, Kamphaengsean Nakhon Pathom 73140, Thailand.
  • Warawidh Wajjwaklu Faculty of Veterinary, Kasetsart University, Kamphaengsean Nakhon Pathom 73140, Thailand.
  • Surachai Saelim Department of Animal Science, Faculty of Agriculture, Kasetsart University, Kamphaengsean Nakhon Pathom 73140, Thailand.
  • Prapant Kessank Faculty of Veterinary, Kasetsart University, Kamphaengsean Nakhon Pathom 73140, Thailand.
  • Lolita Meksongsee Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand

Keywords:

malignant hyperthermia, polymerase chain reaction, halothane test, swine

Abstract

Polymerase chain reaction (PCR) technique was used to detect malignant hyperthermia (MH) genotypes in swine by amplifying the calcium-releasing channel gene with the use of the forward primer (5'-TCCAGTTTGCCACAGGTCCTACCA-3') and the reverse primer (5'-TTCACCGGAGTGGAGTCTCTGAGT-3'). A DNA fragment consisting of 659 base pairs (bp) was obtained. Digestion of this DNA fragment by Hha I yielded two fragments (166 and 493 bp) for the normal genotype (NN), three fragments (166, 493 and 659 bp) for the heterozygous genotype (Nn) and only one fragment (659 bp) for the recessive homozygous genotype (nn). PCR was compared with the halothane test, 190 swines aging from 3-8 weeks were tested. Detecting genotypes by PCR differed from the halothane test by 1.16 percent in the halothane negative (NN or Nn) and by 33.33 percent in the halothane positive (nn) animals.

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Published

1996-12-31

How to Cite

Sinchai Paraksa, Warawidh Wajjwaklu, Surachai Saelim, Prapant Kessank, and Lolita Meksongsee. 1996. “Malignant Hyperthermia in Swine I. Detection Comparing Between PCR and Halothane Technique”. Agriculture and Natural Resources 30 (4). Bangkok, Thailand:429-34. https://li01.tci-thaijo.org/index.php/anres/article/view/241191.

Issue

Vol. 30 No. 4 (1996): October-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.