Effects of EM on the Growth of N2-fixing Bacteria

Abstract

N₂-fixing bacteria, Azotobacter sp. and Azospirillum brasilense were cultured on Brown’s medium (1968) and Dobereiner’s medium (1976) with 1% super EM and without super EM, respectively to evaluate the effect of super EM on the growth of the bacteria, Results found that, the growth of Azotobacter sp grown on Brown’s medium with and without super EM was not significant difference. Reduction of the population growth of Azospirillum brasilense was found when it was grown on Dobereiner’s medium with 1% super EM. More over, contamination existed on the medium with super EM causing inhibitive effect of A. brasilelnse growth.