Screening for Bacterial Wilt Resistant of Tomato

Abstract

Four artificial inoculation methods (Clipping, Stem inoculation, Micropipette and Root inoculation technique) were used for screening bacterial wilt resistance of tomato caused by Pseudomonas solanacearum. The experiments were conducted. The first experiment, nine tomato varieties were inoculated with 1 x 10⁸ cfu/ml of P.solanacearum Nakorn Pathom isolate. No significant differences among the four inoculate methods were found. Cl5915-233D₄-2-1-0 shows resistance, Cl 184 and Cl 5915-206 D₄-2-5-0 moderately resistance and Seedathip 2, Cl 153, Mishou, Seeda, P 502 and VF 134-1-2 moderately susceptible. The second experiment, ten tomato varieties were inoculated with P. solanacearum Nongkai isolate of 1x10⁸ cfu/ml, the same concentration as used in the first experiment. No significant differences between Clipping technique and Stem inoculation technique, Stem inoculation technique and Micropipette technique, Micropipette technique and Root inoculation technique were found. There were significant differences between Clipping technique and Root inoculation technique. No tomato varieties showed any resistance. Cl5915-233 D₄-2-1-0 showed only moderately resistance, Cl 80 moderately susceptible and Cl 110, Cl 115, Cl 116, Cl 159, Cl 162, P 502, Early Pink and 3-31A-B₂ susceptible