Induced Mutation in Rice by Tissue Culture I

Authors

  • P. Pongtongkam Department of Genetics, Factulty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • P. Ratisoontorn Department of Genetics, Factulty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • S. Suputitada Department of Genetics, Factulty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • S. Peyachoknagul Department of Genetics, Factulty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • L. Ngernsiri Department of Genetics, Factulty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • B. Silayoi Department of Horticulture, Factulty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • P. Rodrangboon Department of Genetics, Factulty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • K. Klakhaeng Pathumtani Rice Research Center, Klong Luang, Pathumtani 12120, Thailand.
  • R. Senghaphan Pathumtani Rice Research Center, Klong Luang, Pathumtani 12120, Thailand.

Keywords:

gamma radiation, mutation, rice

Abstract

Seeds of Basmati 370 rice variety were cultured on the modified MS medium supplemented with various concentrations of 2,4-D, NAA and kinetin. The suitable callus induction medium was the modified MS medium containign I mg/l 2,4-D, 2 mg/l NAA and 1 mg/l kinetin. Calli from this medium were divided into two groups, one was 3 week old calli (non subcultured calli) and the other was 6 week old calli (subcultured calli). Both groups seemed to grow and develop into plantles quite well on the modified MS medium supplemented with 2 mg/l kinetin. However the percentage of plantles induction from 3 week old calli was better. Calli of both groups were irradiated with different doses of gamma radiation and were cultured on the regenerated medium. The 3 week old calli were better than 6 week old calli in regenerating into plantles.

Downloads

Published

1994-09-30

How to Cite

P. Pongtongkam, P. Ratisoontorn, S. Suputitada, S. Peyachoknagul, L. Ngernsiri, B. Silayoi, P. Rodrangboon, K. Klakhaeng, and R. Senghaphan. 1994. “Induced Mutation in Rice by Tissue Culture I”. Agriculture and Natural Resources 28 (3). Bangkok, Thailand:381-89. https://li01.tci-thaijo.org/index.php/anres/article/view/241424.

Issue

Vol. 28 No. 3 (1994): July-September

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.