Embryo Culture of Interspecific Hybrid in Brassica spp.

Authors

  • Chuanpis Aroonrungsikul Seed Technology Unit, Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Sumontha Nopparatana Seed Technology Unit, Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Prateang Donsompai Seed Technology Unit, Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.

Keywords:

Brassica spp., interspecific hybrid, embryo rescue, vernalization, electrophoresis

Abstract

Vernalization of inbibed seed of Brassica campestris and B. juncea and one month old plant of B. oleracea were investigated at 5°C, contiouous illumination for 30 and 45 days, respectively. Vernalization procedures and flowering synchronization were adjusted to enhance the flower buds for making interspecies pollination. Then each pot plant was kept on growht cabinet which controlled 23-25°C and continuous light duration. Twenty days after pollination, the hybrid capsules were collected and embryos rescued into the modified MS medium additon with 10% (V/V) coconut juice, 300 mg/l casein hydrolysate, 0.1 gm/l NAA and 0.1 mg/l BA. Thirty days after culture the hybrid embryos between B. campestris x B. oleracea, B. juncea x B. campestris, B. juncea x B. oleracea, B. compestris x B. juncea were regenerated to plantles. Phenotype character of the interspecific hybrids and their parents were examined, the electrophoresis banding pattern of esterase (EST), malate dehydrogenase (MDH), and alcohol dehydrogenase (ADH) pattern were revealed, as well.

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Published

1994-12-31

How to Cite

Chuanpis Aroonrungsikul, Sumontha Nopparatana, and Prateang Donsompai. 1994. “Embryo Culture of Interspecific Hybrid in Brassica Spp”. Agriculture and Natural Resources 28 (4). Bangkok, Thailand:489-98. https://li01.tci-thaijo.org/index.php/anres/article/view/241451.

Issue

Vol. 28 No. 4 (1994): October-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.