Embryo Culture of Interspecific Hybrid in Brassica spp.

Abstract

Vernalization of inbibed seed of Brassica campestris and B. juncea and one month old plant of B. oleracea were investigated at 5°C, contiouous illumination for 30 and 45 days, respectively. Vernalization procedures and flowering synchronization were adjusted to enhance the flower buds for making interspecies pollination. Then each pot plant was kept on growht cabinet which controlled 23-25°C and continuous light duration. Twenty days after pollination, the hybrid capsules were collected and embryos rescued into the modified MS medium additon with 10% (V/V) coconut juice, 300 mg/l casein hydrolysate, 0.1 gm/l NAA and 0.1 mg/l BA. Thirty days after culture the hybrid embryos between B. campestris x B. oleracea, B. juncea x B. campestris, B. juncea x B. oleracea, B. compestris x B. juncea were regenerated to plantles. Phenotype character of the interspecific hybrids and their parents were examined, the electrophoresis banding pattern of esterase (EST), malate dehydrogenase (MDH), and alcohol dehydrogenase (ADH) pattern were revealed, as well.