Plant Regeneration from Callus of Soybean (Glycine max L.)

Abstract

The procedures for plant regeneration from primary leaf and hypocotyl calli fo soybean cv. SJ 4 were developed. The conditions for callus induction from the primary leaf were on B5 medium supplemented with 1-3 mg/l BA, 0.10-0.20 mg/l NAA and 1,650 mg/l NH₄NO₃. The optimum medium for regenerating primary leaf callus were on B5 medium supplemented with 1 mg/l BA, 0.15 mg/l NAA, 1,650 mg/l NH₄NO₃ and the same medium with 2 mg/l BA, 0.15 mg/l NAA and 1,650 mg/l NH₄NO₃. For the hypocotyl a piece located 2 mm. lower from the cotyledonary node can be regenerated. The condition for callus induction from hypocotyl were on RV-5 medium supplemented with 0.1-0.4 mg/l IAA and 0.5-3 mg/l BA. The appropriate medium for regenerating hypocotyl callus was RV5 medium supplemented with 0.5 mg/l BA and 0.2 mg/l IAA.