Complete Nucleotide Sequence and Genome Analysis of Bipartite Tomato Yellow Leaf Curl Virus in Thailand

Authors

  • Supat Attathom Dept. of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand.
  • Pissawan Chiemsombat Dept. of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand.
  • Wichai Kositratana Dept. of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand.
  • Nuchnard Sae-Ung Plant Genetic Engineering Unit, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand.

Keywords:

tomato yellow leaf curl virus, geminiviruses, nucleic acid sequence

Abstract

The genome of Thai-isolate of tomato yellow leaf curl virus (TYLCV-THL), a bipartite geminivirus, was cloned and the nucleotide sequence was analysed. The genome designated component A and B are 2751 and 2737 nucleotides long, respectively. The component A contains seven open reading frames (ORFs) with coding potential for proteins of molecular weight more than 10,000 Daltons. The component B has totally three ORFs on both strands. Computer-based analysis to compare nucleotide sequence of TYLCV-THL DNAs and the predicted amino acids with other selected geminiviruses indicated high degree of similarity with geminivirus which infected tomato in Australia and Israel for the component A. The component B showed greater homology of amino acid sequence with those of cassava latent and squash leaf curl geminiviruses.

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Published

1994-12-31

How to Cite

Supat Attathom, Pissawan Chiemsombat, Wichai Kositratana, and Nuchnard Sae-Ung. 1994. “Complete Nucleotide Sequence and Genome Analysis of Bipartite Tomato Yellow Leaf Curl Virus in Thailand”. Agriculture and Natural Resources 28 (4). Bangkok, Thailand:632-39. https://li01.tci-thaijo.org/index.php/anres/article/view/241478.

Issue

Vol. 28 No. 4 (1994): October-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.