Acaricidal Activity of the Combination of Plant Crude-extracts to Tropical Cattle Ticks

Abstract

Thirty four ethanol crude-extracts of plant species were studied the acaricidal activity of the two combined crude-extracts to the engorged female tropical cattle tick (Boophilus microplus). The concentration of crude-extract was 2.5% in mixed solution of 9 parts of 1% Tween 80^® and 1 part of 100% ethanol. The acaaricidal activity of the combined crude-extracts were tested by dipping method and observed the corrected mortality of the ticks after dipping 24 h, 48 h and 7 days. The acute acaricidal activity were highly found in the combined crude-extracts as follows; Aganonerion polymorphum Pierre ex Spire (Som-lom) with Calotropis procera R.Br. (Rak-dok-khaao), A. polymorphum with Anethum graveolens L.. (Phak-chee-laao); C. procera with Pentapetes Phoenicia L.. (Po-seng). The delayed acaricidal activity were highly wxhibited in the combined crude-extracts of A. polymorphum with Calotropis gigantean R.Br. (Rak-dok-muang), A. polymorphum with Cryptostegia grandiflora R.Br. (Baan-buree-muang), A. polymorphum with lxora nigricans Wight & Arn. (Lum-phuk), C. gigantean with C. grandiflora, C. gigantea with P.phoenicia, I. nigricans with A. graveolens, and Annona muricata L. (Thu-rian-thet) with Piper nigrum L. (Phrik-thai-dam). This results indicated that the practical application of the combination of two crude-extracts of these common plants to control the tropical cattle tick is possible.