Tissue Culture in Rice

Abstract

Tests had been performed to determine the proper condition and most suitable ingredient of medium for 6 culturing different rice varieties. It was found that seeds culture of all six varieties for callus induction grown in the light condition was better than in the dark condition. The suitable callus induction medium for Kaodogmali 105 (KDML 105), Basmati 370 (Bas 370) and Rice Department 15 (RD 15) varieties was the modified MS me¬dium containing 5 mg./l NAA and 1 mg./l kinetin, for Nangmol S4 (NMS 4) and Pradoodaeng (PDD) varieties was the modified MS medium supplemented with 4 mg./l NAA and 1 mg./l kinetin, and for Patumthani 60 (PTT 60) variety was the modified MS medium supplemented with 5 mg./l NAA and 0.5 mg./I kinetin. The suitable plantlet regenerated medium for KDML 105 and NMS4 varieties was the modified MS me¬dium without growth regulator, for PTT 60 varity was the modified MS medium containing 1 mg./l kinetin, for RD 15 variety was the modified MS medium supplemented with 2 mg./l kinetin, and for Bas 370 and PDD varie¬ties was the modified MS medium supplemented with 3 mg./l kinetin