Plant Regeneration from Shoot Tip Culture of Sugarcane

Authors

  • Kriuk Naritoom Dept. of Applied Radiation and Isotopes, Faculty of Science, Kasetsaart University, Bangkok10900, Thailand
  • Kasem Sooksathan Dept. of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
  • Vichai Korpradittskul Dept. of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • Isara Suksathan Dept. of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
  • Rongrong Visessuwan Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Bangkok 10900, Thailand.
  • Siriwan Burikam Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Bangkok 10900, Thailand.
  • Supaporn Klinkong Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Bangkok 10900, Thailand.
  • Monthar Suriyachaiyakorn Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

sugarcane, shoot tip culture, gyratory shaker

Abstract

Sugarcane shoot tips were cultured in liquid media continuously stirred in circular manner by a gyratory shaker rotated at 2 cycles/min under continuous illumination of 2000-4000 lux. It was found that the MS medium supplemented with 0.2 mg/1 BA and 0.02 mg/1 NAA gave the best proliferation rate, 4-5 shootlets could be obtained within 20 days from development of axillary buds. After 50 days the shootlets were multiplied at the rate of 4 folds in every 2 weeks by subculturing into the same medium The resulting shootlets could be progressively increased at the rate of 6 folds in every 4 weeks when transferred on to an MS solid medium, supplemented with 0.2 mg/l BA and 0.02 mg/1 NAA before transferred on to MS medium containing 15oh (v/v) coconut water and 1 mg/ l 2,4-D to enhance the shoots. Rooting of the shoots were successfully done on a MS medium with 1 mg/I IBA.

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Published

1993-09-30

How to Cite

Kriuk Naritoom, Kasem Sooksathan, Vichai Korpradittskul, Isara Suksathan, Rongrong Visessuwan, Siriwan Burikam, Supaporn Klinkong, and Monthar Suriyachaiyakorn. 1993. “Plant Regeneration from Shoot Tip Culture of Sugarcane”. Agriculture and Natural Resources 27 (3). Bangkok, Thailand:286-91. https://li01.tci-thaijo.org/index.php/anres/article/view/241566.

Issue

Vol. 27 No. 3 (1993): July-September

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.