In Vitro Preservation of Sugarcane Plantlets under Low Temperature

Authors

  • Rewat Lersrutaiyotin Dept. of Agronomy, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 75140, Thailand
  • Pongpeng Chit-areerat Dept. of Plant Pathology, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand
  • Rongrong Visessuwan Central Laboratory and Greenhouse Complex, Kasetsart University

Keywords:

sugarcane, in vitro germplasm preservation

Abstract

Plantlets of two cultivated sugarcane cultivars, Supan 1 and CO2, cultured on modified Murashige and Skoog supplemented with 1 mg/l 2,4-dichlorophenoxy acetic acid, 0.1 mg/l myo-inositol, 0.02 mg/l glycine, 20g/l sucrose and 10% by volume of coconut water (CD media) were used. Plantlets were transferred to incubator controlled at 5°C and 15°C under 24 hours light condition for 1 and 2 months. After the storage periods, plantlets were cultured under room temperature for 1 month. Culture under 5°C for 1 month and 15°C for 1 and 2 months could decrease growth of sugarcane plantlets in which all of plantlets could normally rcgrowth under room temperature. Plantlets of sugarcane cultivar Supan 1 cultured under 5°C for 2 months could. regrowth only 50-60% under room temperature.

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Published

1993-12-31

How to Cite

Rewat Lersrutaiyotin, Pongpeng Chit-areerat, and Rongrong Visessuwan. 1993. “In Vitro Preservation of Sugarcane Plantlets under Low Temperature”. Agriculture and Natural Resources 27 (5). Bangkok, Thailand:9-11. https://li01.tci-thaijo.org/index.php/anres/article/view/241732.

Issue

Vol. 27 No. 5 (1993): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.