Production and Verification of Interspecific Hybrids in Brassica spp.

Authors

  • Chuanpis Aroonrungsikull Central Laboratory and Greenhouse Complex, KURDI, Kasetsarl University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Kasem Piluek Dept. of Horticulture, Faculty of Agrilture, Kasetsart University, 10900 Thailand.
  • Pornpan Phuprompunl Central Laboratory and Greenhouse Complex, KURDI, Kasetsarl University, Kamphaeng Saen, Nakhon Pathom 73140,Thailand.
  • Siriwan Burikamt Central Laboratory and Greenhouse Complex, KURDI, Kasetsarl University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Siriporn Chumsangchotsakult Central Laboratory and Greenhouse Complex, KURDI, Kasetsarl University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.

Keywords:

interspecific hybridization, Brassica spp., embryo rescue, electrophoresis

Abstract

Twenty days after cross pollinated Brassica spp. hybrid capsules were collected and embryo rescued into the modified MS. media supplement with 300 mg/l casein hydrolysate, 0.1 mg/l α-naphthaleneacetic acid (NAA) and 0.1 mg/l 6-benzylaminopurine (BA). The efficient production of interspecific hybrids betweenB. campestris x B. juncea, B. JunceaxB. campestris, B. campestris xB. oleracea andBa juncea xB. oleracea were successfully regenerated to plantlets after 14 days in vitro culture. The hybrids were confirmed for fertility of pollen mother cell and the electrophoretic patterns of esterase (EST), malate dehydrogenase (MDII) and alcohol dchydrogenase (ADH) were observed between species by using polyacrylamide gel.

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Published

1993-12-31

How to Cite

Chuanpis Aroonrungsikull, Kasem Piluek, Pornpan Phuprompunl, Siriwan Burikamt, and Siriporn Chumsangchotsakult. 1993. “Production and Verification of Interspecific Hybrids in Brassica Spp”. Agriculture and Natural Resources 27 (5). Bangkok, Thailand:27-36. https://li01.tci-thaijo.org/index.php/anres/article/view/241737.

Issue

Vol. 27 No. 5 (1993): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.