RAPD Analysis for Varietal Identification in Brassica

Authors

  • Panie Temiesak Seed Technology Unit, Central Laboratory and Greenhouse Complex Kasetsart University, Kamphaeng Saen, Nakhon Pathom, Thailand.
  • Yauwanit Ponpim Seed Technology Unit, Central Laboratory and Greenhouse Complex Kasetsart University, Kamphaeng Saen, Nakhon Pathom, Thailand.
  • Takeo Harada Laboratory of Plant Breeding, Hirosaki University, Japan.

Keywords:

RAPD, PCR, Brassica, cultivar identification

Abstract

The concentration of DNA extracted from leaves of Brassica seedlings was amplified by RAPD (Random Amplified Polymorphic DNA) using 35 single 10-base oligonucleotide primers to analyzed genetic relationships among Brassica species. Amplified products were then analyzed by polyacrylamide gel electrophoresis. Each species expressed specific banding pattern. RAPD products were then used to (a) prove genetic basis of an interspeeific hybrid betweenBrassica campestris andB. juncia, and (b) examine differences both within and among parents and hybrids in B. campestris var. pekinensis.

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Published

1993-12-31

How to Cite

Panie Temiesak, Yauwanit Ponpim, and Takeo Harada. 1993. “RAPD Analysis for Varietal Identification in Brassica”. Agriculture and Natural Resources 27 (5). Bangkok, Thailand:37-42. https://li01.tci-thaijo.org/index.php/anres/article/view/241738.

Issue

Vol. 27 No. 5 (1993): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.