Isozymes Electrophoresis for Varietal Verification in Some Tropical Vegetables

Authors

  • Panie Temiesak Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, Thailand.
  • Kasemsak Palakorn Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, Thailand.
  • Karnda Tantiyavarong Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, Thailand.
  • Pornpan Pooprompan Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, Thailand.

Keywords:

electrophoresis, identification, isozymes

Abstract

Techniques for varietal identification of vegetable crops using seed protein and isozymes electrophoresis were investigated. Several isozymes namely, alcohol dehydrogenase (ADH), peroxidase, esterase, glutamic oxaloacetic transaminase (GOT), phosphoglucomutase (PGM) and malate dehydrogenase (MDH) were analysed to standardize in proper condition of plant ages, plant parts, sample extraction solution, gel concentration and staining solution. It had been found that 12 % gel was suitable to analyse isozymes and 13.5 % for protein. Isozymes extracted with phosphate buffer, pH 7.5-8.5 from leaves of 7 day old seedling showed the best expression of banding pattern and distinguish the difference among breeding lines, namely peroxidase in sweet corn, ADH, PGM and esterase in Brassica; esterase in tomato and okra

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Published

1993-12-31

How to Cite

Panie Temiesak, Kasemsak Palakorn, Karnda Tantiyavarong, and Pornpan Pooprompan. 1993. “Isozymes Electrophoresis for Varietal Verification in Some Tropical Vegetables”. Agriculture and Natural Resources 27 (5). Bangkok, Thailand:43-51. https://li01.tci-thaijo.org/index.php/anres/article/view/241739.

Issue

Vol. 27 No. 5 (1993): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.