In vitro Preservation of Sugarcane Callus under Low Temperature

Authors

  • Rewat Lersrutaiyotin Dept. of Agronomy, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Rongrong Visessuwan Central Laboratory and Greenhouse Complex, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Kriuk Naritoom Dept. of Ratio Isotope, Faculty of Science, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.

Keywords:

sugarcane, germplasm preservation, in vitro, low temperature, callus

Abstract

Callus was induced from commercial cultivar of sugarcane by culture shoot tip on Murashige and Skoog medium supplemented with 3 mg/1/2, 4-dichlorophenoxyacetic acid and 15% by volume of coconut water. This callus was preserved under low temperature at 1 and 5 degree C with different periods of preservation, and then was cultured at 27 degree C for 6 months. After preservation under low temperature, plantlets could be induced from callus preserved at 5 degree C but could not be induced from callus preserved at 0 degree C. In addition, at 5 degree C callus could develop to be plantlets only when preserved not longer than 30 days. Callus preserved at low temperature grew slower than callus not preserved at low temperature.

Published

1992-12-31

How to Cite

Rewat Lersrutaiyotin, Rongrong Visessuwan, and Kriuk Naritoom. 1992. “In Vitro Preservation of Sugarcane Callus under Low Temperature”. Agriculture and Natural Resources 26 (5). Bangkok, Thailand:6-9. https://li01.tci-thaijo.org/index.php/anres/article/view/241863.

Issue

Vol. 26 No. 5 (1992): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.