In vitro Preservation of Sugarcane Callus by Using Daminozide

Authors

  • Rongrong Visessuwan Central Laboratory and Greenhouse Complex, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Rewat Lersrutaiyotin Dept. of Agronomy, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.

Keywords:

daminozide, sugarcane, germplasm preservation, in vitro, callus

Abstract

Callus was induced from 2 cultivars and 1 line of sugarcane by culture shoot tip on Murashige and Skoog medium supplemented with 3 mg/l 2, 4-dichlorophenoxy acetic acid and 15% by volume of coconut water. This callus was cultured on MS medium supplemented with 1 mg/l 2, 4-D, 15% by volume of coconut water and various concentration of daminozide for 5 months. Callus cultured on medium with 500 mg/l daminozide could develop to be plantlets, while callus cultured on medium with 1000, 1500 and 2000 mg/l daminozide could not develop to be plantlets. By using daminozide, callus of sugarcane could be stored for 5 months by subculture only 1 time after culture for 2 months.

Published

1992-12-31

How to Cite

Rongrong Visessuwan, and Rewat Lersrutaiyotin. 1992. “In Vitro Preservation of Sugarcane Callus by Using Daminozide”. Agriculture and Natural Resources 26 (5). Bangkok, Thailand:10-12. https://li01.tci-thaijo.org/index.php/anres/article/view/241864.

Issue

Vol. 26 No. 5 (1992): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.