Effect of Growth Suppression Chemicals on In Vitro Preservation of Sugarcane Plantlets
Keywords:
sugarcane, preservation, in vitro, growth suppression chemicals, plantletAbstract
Plantlets of wild and cultivated sugarcane were induced from culture shoot tips on Murashige and Skoog medium with 1 mg/l kinetin, 0.5 mg/l gibberrellic acid (GA3), 1 mg/l indole-3-butylic acid (IBA), 10% by volume of coconut water and 20 g/l sucrose at pH 5.8. The suggested concentration of growth suppression chemicals added in medium for reducing growth of sugarcane plantlets were 3-5% manitol, 20-40 mg/l paclobutrazol and 750-1,000 mg/l mepiquat chloride. Using these chemicals sugarcane plantlets could be maintained under in vitro condition for more than 3 months.
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online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
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